Anti-Osteoporotic Effects of Combined Extract of Lycii Radicis Cortex and Achyranthes japonica in Osteoblast and Osteoclast Cells and Ovariectomized Mice.
Eunkuk ParkJeonghyun KimSubin YeoEunguk LimChun Whan ChoiSangho ChoiWan Yi LiJi-Won LeeJin-Hyok ParkDam HuhSeon-Yong JeongPublished in: Nutrients (2019)
Osteoporosis is characterized by low bone density and quality with high risk of bone fracture. Here, we investigated anti-osteoporotic effects of natural plants (Lycii Radicis Cortex (LRC) and Achyranthes japonica (AJ)) in osteoblast and osteoclast cells in vitro and ovariectomized mice in vivo. Combined LRC and AJ enhanced osteoblast differentiation and mineralized bone-forming osteoblasts by the up-regulation of bone metabolic markers (Alpl, Runx2 and Bglap) in the osteoblastic cell line MC3T3-E1. However, LRC and AJ inhibited osteoclast differentiation of monocytes isolated from mouse bone marrow. In vivo experiments showed that treatment of LRC+AJ extract prevented OVX-induced trabecular bone loss and osteoclastogenesis in an osteoporotic animal model. These results suggest that LRC+AJ extract may be a good therapeutic agent for the treatment and prevention of osteoporotic bone loss.
Keyphrases
- bone loss
- bone mineral density
- postmenopausal women
- induced apoptosis
- bone marrow
- oxidative stress
- bone regeneration
- body composition
- cell cycle arrest
- endoplasmic reticulum stress
- adipose tissue
- signaling pathway
- endothelial cells
- combination therapy
- quality improvement
- high glucose
- mass spectrometry
- dendritic cells
- replacement therapy
- high resolution
- insulin resistance
- smoking cessation
- single molecule
- angiotensin ii