How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?
Robert P PellegrinelliLei YueEduardo CarrascosaStephan WarnkeAhmed Ben FalehThomas R RizzoPublished in: Journal of the American Chemical Society (2020)
Despite the essential role that glycans play in many biological processes, their isomeric complexity makes their structural determination particularly challenging. Tandem mass spectrometry has played a central role in glycan analysis, and recent work has shown that fragments generated by collision-induced dissociation (CID) of disaccharides can retain the anomeric configuration of the glycosidic bond. If this result proves to be general, it would provide a powerful new tool for glycan sequencing. In this work, we use messenger-tagging infrared (IR) spectroscopy to investigate the generality of anomer retention in CID by exploring different fragmentation channels in glycans of increasing complexity. Our results demonstrate that anomericity seems to be retained irrespective of fragment size and branching.
Keyphrases
- cell surface
- tandem mass spectrometry
- high glucose
- diabetic rats
- ultra high performance liquid chromatography
- solid phase extraction
- high performance liquid chromatography
- liquid chromatography
- high resolution
- gas chromatography
- drug induced
- endothelial cells
- oxidative stress
- single molecule
- single cell
- high resolution mass spectrometry
- stress induced