Development of 19F-NMR chemical shift detection of DNA B-Z equilibrium using 19F-NMR.

Shigetaka NakamuraH YangC HirataF KersaudyK Fujimoto

Published in: Organic & biomolecular chemistry (2017)

Various DNA conformational changes are in correlation with biological events. In particular, DNA B-Z equilibrium showed a high correlation with translation and transcription. In this study, we developed a DNA probe containing 5-trifluoromethylcytidine or 5-trifluoromethylthymidine to detect DNA B-Z equilibrium using 19F-NMR. Its probe enabled the quantitative detection of B-, Z-, and ss-DNA based on 19F-NMR chemical shift change.

Keyphrases

circulating tumor
single molecule
cell free
high resolution
magnetic resonance
molecular dynamics
molecular dynamics simulations
solid state
living cells
nucleic acid
circulating tumor cells
quantum dots
real time pcr