Iridium-based probe for luminescent nitric oxide monitoring in live cells.
Chun WuKe-Jia WuTian-Shu KangHui-Min David WangChung-Hang LeungJin-Biao LiuDik-Lung MaPublished in: Scientific reports (2018)
Nitric oxide (NO) is an intra- and extracellular messenger with important functions during human physiology process. A long-lived luminescent iridium(III) complex probe 1 has been designed and synthesized for the monitoring of NO controllably released from sodium nitroprusside (SNP). Probe 1 displayed a 15-fold switch-on luminescence in the presence of SNP at 580 nm. The probe exhibited a linear response towards SNP between 5 to 25 μM with detection limit at 0.18 μM. Importantly, the luminescent switch-on detection of NO in HeLa cells was demonstrated. Overall, complex 1 has the potential to be applied for NO tracing in complicated cellular environment.
Keyphrases
- quantum dots
- nitric oxide
- cell cycle arrest
- induced apoptosis
- sensitive detection
- genome wide
- living cells
- energy transfer
- loop mediated isothermal amplification
- endothelial cells
- high density
- hydrogen peroxide
- endoplasmic reticulum stress
- real time pcr
- nitric oxide synthase
- gene expression
- label free
- pi k akt
- fluorescent probe
- cell proliferation
- signaling pathway
- climate change