Tn6350, a Novel Transposon Carrying Pyocin S8 Genes Encoding a Bacteriocin with Activity against Carbapenemase-Producing Pseudomonas aeruginosa.
Helena TuranoFernando GomesGesiele A Barros-CarvalhoRalf LopesLouise CerdeiraLuis E S NettoAna C GalesNilton LincopánPublished in: Antimicrobial agents and chemotherapy (2017)
A novel transposon belonging to the Tn3-like family was identified on the chromosome of a commensal strain of Pseudomonas aeruginosa sequence type 2343 (ET02). Tn6350 is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS481 family), a transposase (DDE catalytic type), a Tn3 resolvase, three hypothetical proteins, and genes encoding the new pyocin S8 with its immunity protein. We show that pyocin S8 displays activity against carbapenemase-producing P. aeruginosa, including IMP-1, SPM-1, VIM-1, GES-5, and KPC-2 producers.
Keyphrases
- pseudomonas aeruginosa
- acinetobacter baumannii
- klebsiella pneumoniae
- cystic fibrosis
- genome wide
- multidrug resistant
- biofilm formation
- gram negative
- drug resistant
- escherichia coli
- bioinformatics analysis
- minimally invasive
- amino acid
- genome wide identification
- working memory
- copy number
- dna methylation
- gene expression
- staphylococcus aureus
- protein protein
- transcription factor
- crystal structure