Possible COVID-19-Associated Pulmonary Aspergillosis Due to Aspergillus niger in Greece.
Maria KatsiariAngeliki MavroidiEleftheria PallaKonstantina ZourlaTheodoros AlonistiotisKyriakos NtorlisCharikleia NikolaouGeorgia VrioniAthanassios TsakrisPublished in: Antibiotics (Basel, Switzerland) (2022)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes direct damage to the pulmonary epithelium, enabling Aspergillus invasion. Rapid progression and high mortality of invasive aspergillosis have been reported. In the present study, we report a rare case of possible COVID-19-associated pulmonary aspergillosis (CAPA) caused by A. niger in a Greek patient. Diagnosis was based on ECMM/ISHAM specific criteria and the new algorithm "BM-AspICU" for the invasive pulmonary aspergillosis diagnostic strategy. The fungal isolate was recovered in a non-bronchoalveolar lavage (non-BAL) sample and its identification was performed by standard macroscopic and microscopic morphological studies. MALDI-TOF analysis confirmed the identification of A. niger . In addition, galactomannan antigen and Aspergillus real-time PCR testing were positive in the non-BAL sample, while in serum they proved negative. The A. niger isolate showed an MIC for fluconazole ≥128 μg/mL, for itraconazole and posaconazole 0.25 μg/mL, for voriconazole 0.5 μg/mL, for flucytosine 4 μg/mL, for amphotericin B 1 μg/mL, and for all echinocandins (caspofungin, anidulafungin, micafungin) >8 μg/mL. The patient was initially treated with voriconazole; amphotericin B was subsequently added, when a significant progression of cavitation was demonstrated on chest computed tomography. A. niger was not isolated in subsequent samples and the patient's unfavorable outcome was attributed to septic shock caused by a pandrug-resistant Acinetobacter baumannii strain.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- pulmonary hypertension
- coronavirus disease
- acinetobacter baumannii
- rare case
- computed tomography
- case report
- septic shock
- mass spectrometry
- multidrug resistant
- real time pcr
- magnetic resonance imaging
- pseudomonas aeruginosa
- machine learning
- oxidative stress
- deep learning
- candida albicans
- cardiovascular disease
- cystic fibrosis
- image quality
- sensitive detection
- contrast enhanced