Gemykibivirus detection in acute encephalitis patients from Nepal.
Eans Tara TuladharSmita ShresthaSusan VernonLindsay DroitKathie A MihindukulasuriyaMamta TamangLata KarkiAnnie Elong NgonoBimlesh JhaBal Krishna AwalBimal Sharma ChaliseRuna JhaSujan ShrestaDavid WangKrishna Das ManandharPublished in: mSphere (2024)
Viral encephalitis is a devastating disease, but unfortunately, worldwide, the causative virus in many cases is unknown. Therefore, it is important to identify viruses that could be responsible for cases of human encephalitis. Here, using metagenomic sequencing of CSF, we identified a gemykibivirus in a male child from Nepal with acute encephalitis syndrome (AES). We subsequently detected gemykibivirus DNA in CSF or serum of 12 more encephalitis patients by real-time PCR. The virus genomes we identified are highly similar to gemykibiviruses previously detected in CSF of three encephalitis patients from Sri Lanka. These results raise the possibility that gemykibivirus could be an underrecognized human pathogen.
Keyphrases
- end stage renal disease
- newly diagnosed
- ejection fraction
- chronic kidney disease
- endothelial cells
- peritoneal dialysis
- prognostic factors
- patient reported outcomes
- hepatitis b virus
- case report
- patient reported
- single cell
- microbial community
- acute respiratory distress syndrome
- antibiotic resistance genes
- mechanical ventilation
- sensitive detection
- label free