High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for Mycobacterium tuberculosis RNA polymerase.

Drake JensenAna Ruiz ManzanoMaxwell RectorEric J TomkoM Thomas RecordEric A Galburt

Published in: bioRxiv : the preprint server for biology (2023)

steady-state transcription kinetics. We illustrate how an RNA-aptamer-based detection system can be used to generate quantitative information on direct mechanisms of transcriptional regulation and discuss the far-reaching implications for future applications.

Keyphrases

label free
mycobacterium tuberculosis
high throughput
sensitive detection
gold nanoparticles
transcription factor
loop mediated isothermal amplification
quantum dots
magnetic nanoparticles
current status
pulmonary tuberculosis
high resolution
single cell
health information
living cells
real time pcr
social media