Rapid visual detection of benzimidazole resistance in Botrytis cinerea by recombinase polymerase amplification combined with a lateral flow dipstick.

Published in: Pest management science (2021)

This study developed a novel assay based on RPA-LFD for the rapid and equipment-free detection of MBC-resistant isolates. In combination with a simple DNA extraction method, the assay could detect B. cinerea MBC-resistant isolates even without specialized equipment within 30 min. Considering its specificity, stability and simplicity, the RPA-LFD assay could be a promising tool for rapid on-site diagnosis of fungicide-resistant isolates.

Keyphrases

loop mediated isothermal amplification
sensitive detection
high throughput
genetic diversity
palliative care
molecular docking
single molecule
label free
cell free
quantum dots
single cell
structural basis
molecular dynamics simulations