DNA Computation-Modulated Self-Assembly of Stimuli-Responsive Plasmonic Nanogap Antennas for Correlated Multiplexed Molecular Imaging.
Jing ChenDan LiTingting ZhaoJunhao WangJiaheng ShiShuwei ChenYue YinShenghao XuXi-Liang LuoPublished in: Analytical chemistry (2022)
Nanogap antennas with strong electromagnetic fields of the "hot spot" in the gap region of two adjacent particles that can significantly improve the optical properties of fluorophores hold great potential for ultrasensitive bioanalysis. Herein, a DNA computation-mediated self-assembly of Au NBP dimer-based plasmonic nanogap antennas was designed for imaging of intracellular correlated dual disease biomarkers. It is worth noting that with the benefit from the electromagnetic fields of the "hot spot" in the gap region and strand displacement amplification, the fluorescence intensity can be enhanced ∼14.7-fold by Au NBP dimer-based plasmonic nanogap antennas. In addition, the AND-gate sensing mechanism was confirmed through monitoring the response of three designed nAP-PH1, m-PH1, and PH1 probes, the fluorescence recovery in different cell lines (Hela and L02), and inhibitor-treated cells, respectively. Furthermore, thanks to the "dual keys" activation design, such an "AND-gate" sensing manner can be used for ultrasensitive correlated multiplexed molecular imaging, demonstrating its feasible prospect in correlated multiplexed molecular imaging.
Keyphrases
- single molecule
- label free
- energy transfer
- single cell
- living cells
- cell cycle arrest
- quantum dots
- gold nanoparticles
- sensitive detection
- induced apoptosis
- nucleic acid
- high frequency
- reduced graphene oxide
- molecularly imprinted
- cancer therapy
- cell death
- circulating tumor
- reactive oxygen species
- risk assessment
- fluorescence imaging
- endoplasmic reticulum stress
- mass spectrometry
- pi k akt
- cell proliferation