A simple quantitative PCR assay to determine TRAMP transgene zygosity.

Ruidong ChenXin LiangMollianne M MurrayEllen KarasikJenny J HanMing ZhuBarbara A Foster Daniel E Frigo Guocan Wang

Published in: Prostate cancer and prostatic diseases (2020)

We develop the first straightforward, qPCR approach to reliably determine the TRAMP transgene zygosity. The development of this qPCR-based genotyping method enables researchers to streamline breeding strategies when creating complex genetic mouse models involving TRAMP mice; thus, ultimately reducing the required animal numbers, cost, and investigator time.

Keyphrases

high throughput
genome wide
mouse model
high fat diet induced
high resolution
dna methylation
adipose tissue
type diabetes
mass spectrometry
wild type