Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor.
Mohamed Amine BerkalJean-Jacques ToulmeCorinne NardinPublished in: Analytical and bioanalytical chemistry (2024)
Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R 2 ) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.
Keyphrases
- label free
- human health
- loop mediated isothermal amplification
- sensitive detection
- risk assessment
- quantum dots
- climate change
- escherichia coli
- single molecule
- real time pcr
- gold nanoparticles
- magnetic resonance imaging
- hydrogen peroxide
- nitric oxide
- multidrug resistant
- pseudomonas aeruginosa
- staphylococcus aureus
- magnetic resonance
- contrast enhanced
- machine learning
- deep learning
- klebsiella pneumoniae
- molecularly imprinted
- tandem mass spectrometry
- life cycle