Position-specific carbon isotope analysis of serine by gas chromatography/Orbitrap mass spectrometry, and an application to plant metabolism.
Elise B WilkesAlex L SessionsSarah S ZeichnerBrooke DallasBrian SchubertA Hope JahrenJohn M EilerPublished in: Rapid communications in mass spectrometry : RCM (2022)
This study presents the first Orbitrap-based measurements of natural-abundance, position-specific carbon isotope variation in an amino acid isolated from a biological matrix. We present a method for the precise characterization of isotope ratios in serine and propose applications probing metabolism in plants. We discuss the potential for extending these approaches to other amino acids, paving the way for novel applications.
Keyphrases
- gas chromatography
- mass spectrometry
- amino acid
- tandem mass spectrometry
- high resolution mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- high performance liquid chromatography
- gas chromatography mass spectrometry
- high resolution
- capillary electrophoresis
- solid phase extraction
- protein kinase
- simultaneous determination
- human health
- microbial community