Structural characteristics of polysaccharide microcapsules fromNostoc commune, and their applications in skin wound healing and pathological repair.

In this study, the extraction conditions of Nostoc commune Vauch polysaccharide (NCVP) were optimized by single factor and orthogonal experiments. Then, the microcapsules (NCVPM) was prepared. After analyzing the microcapsule structural, thermal characteristics, the skin wound healing ability was studied by establishing back trauma rat models. Results showed that, the NCVP yield was 10.37% under the following optimum conditions: 210 minute extraction time, solid-liquid ratio of 1:50 and extraction temperature of 90°C. The overall performance of the microcapsule was the best when the concentration of sodium alginate, calcium chloride and chitosan was 2%, 3% and 0.3%, respectively. NCVPM had spherical morphology, typical microcapsule structural characteristics and good thermal stability, and NCVP was dispersed in the microcapsules. NCVPM showed good biocompatibility and biodegradability, which met the requirements for slow-release polymer materials. After 14 days treatment, the wound healing rate was 92.4%, the cells were arranged neatly and regularly, the cell nucleus became large and elliptical, and the cell has a tendency to divide, the fibers and microvessel were significantly more. By evaluating mechanism, NVCPM could increase the contents of hydroxyproline (HYP) and glutathione (GSH-Px) to protect cells from oxidative damage, leading in turn to accelerate wound healing and shorted wound healing times. And could accelerate cell division, collagen and microvascular production by increasing transcription levels of Vascular endothelial growth factor (VEGF) mRNA and miRNA-21.