The microbiological outcomes of culture-negative blood specimens using 16S rRNA broad-range PCR sequencing: a retrospective study in a Canadian province from 2018 to 2022.
Anthony LieuLuke B HarrisonJosée HarelAlexander LawandiMatthew P ChengMarc-Christian DomingoPublished in: Journal of clinical microbiology (2024)
Broad-range 16S rRNA PCR and sequencing of 1,183 blood specimens from 853 unique patients yielded an interpretable sequence and bacterial identification in 29%, 16S rRNA amplification with uninterpretable sequences in 53%, and no amplification in 18%. This study highlights the potential utility of this technique in identifying fastidious gram-negative and anaerobic bacteria but the frequent recovery of environmental and contaminant organisms argues for its judicious use.IMPORTANCEThe existing literature focuses on its performance compared to blood cultures in patients with sepsis, leaving a gap in the literature regarding other blood specimens in suspected infectious syndrome across the severity spectrum. We aimed to characterize its microbiological outcomes and provide insight into its potential clinical utility.
Keyphrases
- gram negative
- multidrug resistant
- systematic review
- end stage renal disease
- intensive care unit
- microbial community
- single cell
- newly diagnosed
- acute kidney injury
- chronic kidney disease
- risk assessment
- pulmonary embolism
- type diabetes
- south africa
- metabolic syndrome
- nucleic acid
- peritoneal dialysis
- insulin resistance
- adipose tissue
- human health
- patient reported outcomes
- skeletal muscle
- septic shock
- ultrasound guided