An In Vitro Evaluation of the Hierarchical Micro/Nanoporous Structure of a Ti3Zr2Sn3Mo25Nb Alloy after Surface Dealloying.

A process to dealloy a Ti-3Zr-2Sn-3Mo-25Nb (TLM) titanium alloy to create a porous surface structure has been reported in this paper aiming to enhance the bioactivity of the alloy. A simple nanoporous topography on the surface was produced through dealloying the as-solution treated TLM alloy. In contrast, dealloying the as-cold rolled alloy created a hierarchical micro/nanoporous topography. SEM and XPS were performed to characterize the topography and element chemistry of both porous structures. The roughness, hydrophilicity, protein adsorption, cell adhesion, proliferation, and osteogenic differentiation were tested. The elements of Zr, Mo, Sn, and Nb were depleted at the nanoporous TLM surface with a diameter of 15.6 ± 2.3 nm. Dissolving the microscale α phase from the alloy surface contributed to the formation of the microscale grooves on the surface. The simple nanoporous topographical surface exhibited hydrophilicity and higher protein adsorption ability, which facilitated the early adhesion of osteoblasts compared with the hierarchical micro/nanoporous surface. On the other hand, the hierarchical micro/nanoporous surface improved cell proliferation and differentiation and still retained the contact guidance function, which implied good bonding for osseointegration. This research revealed the effect of phase composition on the surface morphology of dealloying titanium alloy and the synergistic effect of micron and nanometer topography on the function of osteoblasts. This paper therefore provides insights into the surface topological design of titanium-based biomaterials with improved biocompatibility.