Comparison between next-generation sequencing and multiplex polymerase chain reaction assays for nonsmall-cell lung cancer molecular diagnosis.

Shuji MurakamiKanako ShinadaYuka OtsutsumiFumiko KomineYuan YuanJunko NakamuraSeigo KatakuraTetsuro KondoTerufumi KatoTomoyuki YokoseHaruhiro Saito

Published in: Cancer medicine (2024)

The AmoyDx PLC panel, a PCR-based multiplex diagnostic test, exhibits a high success rate. The frequency of the nine genes targeted for treatment detected by the AmoyDx PLC panel was comparable to the frequency of mutations detected by ODxTT-M. Clinicians should understand and use the AmoyDx PLC panel and ODxTT-M with respect to their respective performances and limitations.

Keyphrases

high throughput
real time pcr
single cell
cell therapy
epidermal growth factor receptor
stem cells
gene expression
bone marrow
dna methylation