Development and Validation of an RP-HPLC-PDA Method for Determination of Paracetamol, Caffeine and Tramadol Hydrochloride in Pharmaceutical Formulations.
Fernando J PereiraAida Rodríguez-CorderoRoberto LópezLuis C RoblesA Javier AllerPublished in: Pharmaceuticals (Basel, Switzerland) (2021)
Paracetamol (acetaminophen) (PAR), caffeine (CAF) and tramadol hydrochloride (TRA) are important drugs widely used for many clinical purposes. Determination of their contents is of the paramount interest. In this respect, a quick, simple and sensitive isocratic RP-HPLC method with photodiode array detection was developed for the determination of paracetamol, caffeine and tramadol in pharmaceutical formulations. An improved sensitive procedure was also evolved for tramadol using a fluorescence detector system. A C18 column and a mobile phase constituted by methanol/phosphate were used. LODs were found to be 0.2 μg/mL, 0.1 μg/mL and 0.3 μg/mL for paracetamol, caffeine and tramadol hydrochloride, respectively, using photodiode-array detection. Alternatively, LOD for tramadol decreased to 0.1 μg/mL with the fluorescence detector. Other notable analytical figures of merit include the linear concentration ranges, 0.8-270 μg/mL, 0.4-250 μg/mL and 1.0-300 (0.2-40) μg/mL, for the same ordered analytes (including the fluorescence detector). The proposed method was successfully applied for the quantitative determination of the three drugs in tablet dosage forms.
Keyphrases
- solid phase extraction
- molecularly imprinted
- high performance liquid chromatography
- ms ms
- simultaneous determination
- single molecule
- high resolution
- liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- energy transfer
- magnetic resonance imaging
- anti inflammatory drugs
- magnetic resonance
- real time pcr
- loop mediated isothermal amplification
- high density
- neural network