Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system.

Sin-Yeang TeowKitson LiewMohd Firdaus Che MatMarini MarzukiNorazlin Abdul AzizTai-Lin ChuMunirah AhmadAlan Soo-Beng Khoo

Published in: BMC biotechnology (2019)

XenoLuc assay is specific, sensitive, rapid and cost-effective for measuring the growth of luciferase-expressing cells in a co- or multiple-culture system. This assay may also be adapted for tumour microenvironment studies as well as drug screening experiments in more complex 3D co-culture systems.

Keyphrases

induced apoptosis
cell proliferation
high throughput
cell cycle arrest
stem cells
endoplasmic reticulum stress
signaling pathway
emergency department
cell cycle
pi k akt
single cell
drug induced
adverse drug