Login / Signup

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil.

Nicolas KiefferPatrice NordmannAndrea Micke MorenoLuisa Zanolli MorenoRichard ChabyAude BretonPierre TissièresLaurent Poirel
Published in: Antimicrobial agents and chemotherapy (2018)
A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C2 plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase.
Keyphrases
  • escherichia coli
  • klebsiella pneumoniae
  • multidrug resistant
  • biofilm formation
  • genome wide
  • copy number
  • risk assessment
  • immune response
  • crispr cas
  • drug resistant
  • single cell
  • candida albicans