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A ratiometric fluorescent dye for detection of Lys and Arg and its bioimaging in live cells and zebrafish larvae.

Tongxin ZhangZhengfei YangXiaoyan HeLuxin GuoJincheng WangXuewen JiangRuling ShenXiuhong Lu
Published in: Analytical methods : advancing methods and applications (2023)
A ratiometric and pH-sensitive fluorescent dye named IDE was applied to the detection of argine and lysine from common amino acids and exploited to monitor the Lys and Arg levels in living cells and zebrafish larvae successfully. IDE will be a useful fluorescence indicator of pH changes by Lys and Arg.
Keyphrases
  • living cells
  • fluorescent probe
  • single molecule
  • amino acid
  • loop mediated isothermal amplification
  • induced apoptosis
  • label free
  • real time pcr
  • aedes aegypti
  • highly efficient
  • cell cycle arrest
  • nitric oxide