Login / Signup

Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective.

Caroline Mwendwa KijogiChristopher Khayeka-WandabwaKeita SasakiYoshimasa TanakaHiroshi KurosuHayato MatsunagaHiroshi Ueda
Published in: BMC physiology (2016)
Truncated PTMA could not be detected by western blotting when both antibodies were applied in all tissues examined except the thymus. However, immunohistochemistry revealed differential staining by these antibodies suggesting possible masking of epitopes by interacting molecules. The differential localization patterns observed in the context of nucleic versus cytoplasmic presence as well as punctate versus diffuse pattern in tissues and cell types, warrant further investigations as to the forms of PTMA interacting partners.
Keyphrases
  • single cell
  • gene expression
  • south africa
  • protein kinase
  • stem cells
  • cell therapy
  • flow cytometry