Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus .
Shu WangWanzhu ShenShuai ZhengZhigang LiChongwen WangLong ZhangYong LiuPublished in: RSC advances (2021)
This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO 2 -Au-QD tags for sensitive and quantitative detection of Staphylococcus aureus ( S. aureus ). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method for S. aureus can reach 10 4 and 100 cells mL -1 , respectively.
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- quantum dots
- staphylococcus aureus
- label free
- high throughput
- methicillin resistant staphylococcus aureus
- gold nanoparticles
- induced apoptosis
- living cells
- real time pcr
- high resolution
- biofilm formation
- cystic fibrosis
- single cell
- oxidative stress
- single molecule
- fluorescent probe
- nitric oxide
- cell death
- mass spectrometry
- magnetic nanoparticles