A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity.
Abu Ali Ibn SinaMatthew Thomas FosterDarren KorbieLaura G CarrascosaMohammad J A ShiddikyJing GaoShuvashis DeyKevin M KooPublished in: The Analyst (2017)
We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl-1 of DNA with no sequencing requirement.
Keyphrases
- real time pcr
- dna methylation
- circulating tumor
- cell free
- genome wide
- single molecule
- gene expression
- high throughput
- label free
- single cell
- copy number
- silver nanoparticles
- gold nanoparticles
- loop mediated isothermal amplification
- circulating tumor cells
- ionic liquid
- biofilm formation
- pseudomonas aeruginosa
- escherichia coli
- capillary electrophoresis
- staphylococcus aureus
- reduced graphene oxide
- cystic fibrosis
- electron transfer