Composition and Charge Compensation in Chitosan/Gum Arabic Complex Coacervates in Dependence on pH and Salt Concentration.

In this study, complex coacervates of the biopolyelectrolytes chitosan and gum arabic were investigated with respect to their composition and charge compensation depending on the pH and salt concentration. Individual polyelectrolyte yields were deduced from thermogravimetric analysis and chitosan quantification via enzymatic hydrolysis/HPLC-ELSD. The polyelectrolyte mass ratio in the complex coacervate is found to remain approximately constant irrespective of the pH, despite the latter's effect on the polyelectrolyte charge ratio. Two regimes are identified, including either chitosan charges in excess (at pH < 6.0) or gum arabic charges in excess (at pH > 6.0). The amount of extrinsic charge compensation in the complex coacervates is discussed in detail. We show for the first time that the doping level, a quantity traditionally used to describe salt-induced changes of the charge compensation in polyelectrolyte complexes, is also suitable for the description of pH-induced extrinsic charge compensation in such systems.