Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.

Nidhi GuptaXinhua WangXiaohui WenPaul MoranMaciej PaluchPhilip E HassAmy HeidersbachBenjamin HaleyDaniel KirchhoferRandall J BrezskiAndrew S PetersonSuzie J Scales

Published in: Journal of the American Society of Nephrology : JASN (2020)

Our antibodies highlighted different conformations of native APOL1 topology in serum (HDL particles) and at the podocyte surface. Our findings support the surface ion channel model for APOL1 risk variant-mediated podocyte injury, as well as providing domain accessibility information for designing APOL1-targeted therapeutics.

Keyphrases

diabetic nephropathy
high glucose
small molecule
genome wide
gene expression
cancer therapy
healthcare
health information
dna methylation