Vertical Organic Electrochemical Transistors and Electronics for Low Amplitude Micro-Organ Signals.
Myriam AbarkanAntoine PirogDonnie MafilazaGaurav PathakGilles N'KaouaEmilie PuginierRodney O'ConnorMatthieu RaouxMary Jocelyn DonahueSylvie RenaudJochen LangPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2022)
Electrical signals are fundamental to key biological events such as brain activity, heartbeat, or vital hormone secretion. Their capture and analysis provide insight into cell or organ physiology and a number of bioelectronic medical devices aim to improve signal acquisition. Organic electrochemical transistors (OECT) have proven their capacity to capture neuronal and cardiac signals with high fidelity and amplification. Vertical PEDOT:PSS-based OECTs (vOECTs) further enhance signal amplification and device density but have not been characterized in biological applications. An electronic board with individually tuneable transistor biases overcomes fabrication induced heterogeneity in device metrics and allows quantitative biological experiments. Careful exploration of vOECT electric parameters defines voltage biases compatible with reliable transistor function in biological experiments and provides useful maximal transconductance values without influencing cellular signal generation or propagation. This permits successful application in monitoring micro-organs of prime importance in diabetes, the endocrine pancreatic islets, which are known for their far smaller signal amplitudes as compared to neurons or heart cells. Moreover, vOECTs capture their single-cell action potentials and multicellular slow potentials reflecting micro-organ organizations as well as their modulation by the physiological stimulator glucose. This opens the possibility to use OECTs in new biomedical fields well beyond their classical applications.
Keyphrases
- single cell
- rna seq
- gold nanoparticles
- type diabetes
- induced apoptosis
- cardiovascular disease
- heart failure
- nucleic acid
- high resolution
- high throughput
- high glucose
- ionic liquid
- cell proliferation
- heart rate
- signaling pathway
- spinal cord
- metabolic syndrome
- cell therapy
- blood pressure
- blood glucose
- glycemic control
- stem cells
- adipose tissue
- cell death
- diabetic rats
- mesenchymal stem cells
- water soluble
- endothelial cells
- skeletal muscle
- resting state
- insulin resistance
- spinal cord injury
- weight loss
- atrial fibrillation
- high intensity
- tandem mass spectrometry
- liquid chromatography
- functional connectivity