Polyhydric Stigmastane-Type Steroids Derivative from Vernonia amygdalina and Their Anti-Neuroinflammatory Activity.
Xiangzhong LiuMi ZhouShoulun HeQiannan XuChunchun DuHonghong ZhuTing LinGuanghui WangWenjing TianHai-Feng ChenPublished in: Pharmaceuticals (Basel, Switzerland) (2022)
Vernonia amygdalina Del. is a traditional medicinal plant and vegetable originating from tropical Africa. The phytochemical investigation of V. amygdalina led to eight undescribed polyhydric stigmastane-type steroids, vernonin M-T ( 1 - 8 ). Their gross structures and stereochemistry were elucidated by HR-ESI-MS, 1D and 2D NMR spectra, X-ray diffraction, quantum chemical computation of the ECD spectrum, and the in situ dimolybdenum CD method. The anti-neuroinflammatory activity of the isolated compounds was performed in BV-2 microglia cells. As a result, compound 1 displayed a notable anti-neuroinflammatory effect via suppressing the LPS-induced IκB degradation and restricting the activation of the PI3K/AKT and p38 MAPK pathways.
Keyphrases
- lps induced
- inflammatory response
- high resolution
- ms ms
- induced apoptosis
- mass spectrometry
- lipopolysaccharide induced
- multiple sclerosis
- signaling pathway
- molecular dynamics
- cell cycle arrest
- magnetic resonance imaging
- oxidative stress
- cell death
- density functional theory
- electron microscopy
- quantum dots
- children with cerebral palsy