Bead-beating assay during synovial fluid DNA extraction improves real-time PCR accuracy for periprosthetic joint infection.

Yuta Hieda Hyonmin Choe Hiroyuki Ike Koki AbeKen KumagaiMasanobu TakeyamaYusuke Kawabata Naomi Kobayashi Yutaka Inaba

Published in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (2024)

Polymerase chain reaction (PCR)-based genetic diagnosis is a rapid and sensitive method to diagnose periprosthetic joint infection (PJI). DNA extraction using bead beating is an effective method for collecting bacterial genes in Gram-positive bacteria. We compared the detection accuracy between the conventional and bead-beating DNA extraction assay. The detection rate improved from 86.7% using the conventional method to 95.6% using the bead-beating. Our results suggest that bead-beating during DNA extraction can improve the accuracy of PCR-based genetic diagnosis of PJI.

Keyphrases

real time pcr
circulating tumor
cell free
single molecule
genome wide
loop mediated isothermal amplification
high throughput
nucleic acid
gene expression
circulating tumor cells
copy number
total hip arthroplasty
transcription factor
gram negative
quantum dots