Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice.
Metin YildirimMehmet ErsatirUlas DeğirmenciAkın YiginMustafa Boyraz UnalMuhammet Emin GuldurOnur DemirkolElife Sultan GirayPublished in: International journal of environmental health research (2022)
In this study, the protective effects of Ruta chalepensis L . extracts on the extent of tissue damage in gentamicin-induced nephrotoxicity have been investigated. Ruta chalepensis L . extracts were prepared by subcritical water and ultrasound-assisted organic solvent extraction methods. Protective activity of Ruta chalepensis L . extracts on Gentamicin-induced nephrotoxicity is investigated by apoptotic, DNA damage, oxidative stress markers and evaluating histopathological in kidney tissue of mice. Gentamicin significantly increased Caspase-3 and -8 activities, NO levels, serum creatinine and BUN, while 8-OHdG and MDA levels were significantly decreased with Ruta chalepensis L. extract treatment. In addition, Ruta chalepensis L . extracts treatment significantly increased CAT and SOD activities. Histopathological alterations in Gentamicin group were significantly diminished by application of Ruta chalepensis L . extracts. These results suggest that treatment with Ruta chalepensis L . extracts may ameliorate renal dysfunction and structural damage through the reduction of oxidative stress and apoptosis in the kidney.
Keyphrases
- oxidative stress
- diabetic rats
- dna damage
- induced apoptosis
- cell death
- ischemia reperfusion injury
- high glucose
- drug induced
- endothelial cells
- dna repair
- combination therapy
- adipose tissue
- cell cycle arrest
- mass spectrometry
- skeletal muscle
- climate change
- smoking cessation
- insulin resistance
- breast cancer cells
- stress induced
- replacement therapy