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Ultrafast Detection of Peroxynitrite in Parkinson's Disease Models Using a Near-Infrared Fluorescent Probe.

Qian SunJiajia XuChonglei JiMazen S S ShaibaniZheng LiKahleong LimChengwu ZhangLin LiZhipeng Liu
Published in: Analytical chemistry (2020)
Parkinson's disease (PD) is a neurodegenerative disease that devastatingly affects people's lives. Numerous research studies have shown that peroxynitrite (ONOO-) plays a pivotal role in the pathogenesis of PD. However, a suitable tool that could quickly and sensitively detect ONOO- in various PD models is still lacking. To this end, we designed and synthesized a series of near-infrared probes that could detect ONOO- within seconds by near-infrared fluorescent imaging in an ultrafast and highly selective manner. It is noteworthy that one of those developed probes, NIR-PN1, showed excellent sensing performance and blood-brain barrier penetrating ability. NIR-PN1 was successfully applied for imaging of ONOO- fluxes in multiple PD models including PC12 cell, Drosophila, C. elegans, and mouse brain, indicating its great potential application not only for understanding the biological roles that ONOO- played in PD but also for early PD diagnosis and treatment.
Keyphrases
  • fluorescent probe
  • living cells
  • blood brain barrier
  • fluorescence imaging
  • high resolution
  • small molecule
  • single molecule
  • single cell
  • label free