Harmonization and standardization of nucleus pulposus cell extraction and culture methods.
Shaghayegh BasatvatFrances C BachMarcos N BarcellonaAbbie L BinchConor Timothy BuckleyBrian T BuenoNadeen O ChahineAna V CheeLaura B CreemersStefan DudliBailey V FearingStephen J FergusonJennifer GansauBenjamin GantenbeinRahul GawriJuliane D GlaeserSibylle GradJulien GuerreroLisbet HaglundPaula A HernandezJudith A HoylandC-Y Charles HuangJames C IatridisSvenja Illien-JüngerLiufang JingPetra KrausLisanne T LaaglandGernot LangVictor Y L LeungZhen LiThomas LufkinJosette C van MaanenEmily E McDonnellChris J PanebiancoSteven M PresciuttiSanjna RaoStephen M RichardsonSarah RomereimTara C SchmitzJordy ScholLori SettonDmitriy SheynJoseph W SnuggsY SunXiaohong TanMarianna A TryfonidouNam N VoDong WangBrandon WilliamsRebecca WilliamsS Tim YoonChristine Lyn Le MaitrePublished in: JOR spine (2023)
This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60-100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide.