Determination of IC 50 values of anticancer drugs on cells by D 2 O - single cell Raman spectroscopy.

Wanxue LvBoqiang FuManli LiYu KangShouli BaiChao Lu

Published in: Chemical communications (Cambridge, England) (2022)

A simple, sensitive and repeatable D 2 O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC 50 values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays.

Keyphrases

raman spectroscopy
single cell
induced apoptosis
cell cycle arrest
high throughput
rna seq
mass spectrometry
cell proliferation
drug induced
liquid chromatography