Beta-Blocker Separation on Phosphodiester Stationary Phases-The Application of Intelligent Peak Deconvolution Analysis.
Oktawia KaliszMikołaj DembekSylwia StudzińskaSzymon BocianPublished in: Molecules (Basel, Switzerland) (2023)
Beta-blockers are a class of medications predominantly used to manage abnormal heart rhythms. They are also widely used to treat high blood pressure. From the liquid chromatography separation point of view, beta-blockers are interesting molecules due to their hydrophobic-hydrophilic properties. Thus, the study aimed to investigate the beta-blocker separation selectivity on four phosphodiester stationary phases in reversed-phase liquid chromatography (RP LC) and hydrophilic interactions liquid chromatography (HILIC). On tested stationary phases, beta-blockers provide retention in both chromatographic systems, RP LC and HILIC. Additionally, it was found that cation-exchange mechanisms have a significant contribution to retention. Separations were enhanced by applying ChromSword software for gradient optimization and Intelligent Peak Deconvolution Analysis to separate unseparated peaks digitally.
Keyphrases
- liquid chromatography
- mass spectrometry
- high resolution mass spectrometry
- simultaneous determination
- tandem mass spectrometry
- angiotensin converting enzyme
- blood pressure
- solid phase extraction
- gas chromatography
- capillary electrophoresis
- heart failure
- angiotensin ii
- high resolution
- heart rate
- ionic liquid
- weight loss
- hypertensive patients
- data analysis
- metabolic syndrome