Luminescent Assay for the Screening of SARS-CoV-2 M Pro Inhibitors.

Daan SondagJona MerxEmiel RossingThomas J Boltje Dennis W P M LöwikFrank H T NelissenMark van GeffenCornelis van 't VeerWaander L van HeerdeFloris P J T Rutjes

Published in: Chembiochem : a European journal of chemical biology (2022)

Since the outbreak of SARS-CoV-2 in December 2019 millions of infections have been reported globally. The viral chymotrypsin-like main protease (M Pro ) exhibits a crucial role in viral replication and represents a relevant target for antiviral drug development. In order to screen potential M Pro inhibitors we developed a luminescent assay using a peptide based probe containing a cleavage site specific for M Pro . This assay was validated showing IC 50 values similar to those reported in the literature for known M Pro inhibitors and can be used to screen new inhibitors.

Keyphrases

sars cov
high throughput
anti inflammatory
quantum dots
respiratory syndrome coronavirus
risk assessment
transcription factor
living cells
single molecule