A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum.
Shurong TangXiuhua YouQuanhui FangXin LiGuangwen LiJinghua ChenHuaping PengPublished in: Sensors (Basel, Switzerland) (2019)
A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS₂ QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO₂ NSs) were prepared by exfoliating the bulk δ-MnO₂ material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS₂ QDs could be quenched in the presence of MnO₂ NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO₂ NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20⁻2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.
Keyphrases
- quantum dots
- fluorescent probe
- loop mediated isothermal amplification
- energy transfer
- sensitive detection
- living cells
- single molecule
- label free
- aqueous solution
- real time pcr
- high throughput
- reduced graphene oxide
- high resolution
- highly efficient
- gold nanoparticles
- high intensity
- electron transfer
- anaerobic digestion
- municipal solid waste