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Modular control of l-tryptophan isotopic substitution via an efficient biosynthetic cascade.

Clayton M ThompsonAllwin D McDonaldHanming YangSilvia CavagneroAndrew R Buller
Published in: Organic & biomolecular chemistry (2021)
Isotopologs are powerful tools for investigating biological systems. We report a biosynthetic-cascade synthesis of Trp isotopologs starting from indole, glycine, and formaldehyde using the enzymes l-threonine aldolase and an engineered β-subunit of tryptophan synthase. This modular route to Trp isotopologs is simple and inexpensive, enabling facile access to these compounds.
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