Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry.
Damir OrosMarina CeprnjaJurica ZuckoMario CindricAmela HozicJasenka SkrlinKarmela BarisicEna MelvanKsenija UroicBlazenka KosAntonio StarcevicPublished in: Clinical proteomics (2020)
Our findings indicate that mass spectrometry has great potential as a reliable high-throughput tool for microbial pathogen identification in human urine samples. In this case, the MALDI-TOF/TOF, was used as an analytical tool for the determination of bacteria in urine samples, and the results obtained emphasize high importance of storage conditions and sample preparation method impacting reliability of MS2 data analysis. The proposed method is simple enough to be utilized in existing clinical settings and is highly suitable for suspected single organism infectious etiologies. Further research is required in order to identify pathogens in polymicrobial urine samples.
Keyphrases
- mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- gas chromatography
- high performance liquid chromatography
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- solid phase extraction
- data analysis
- high resolution
- capillary electrophoresis
- high throughput
- simultaneous determination
- ms ms
- molecularly imprinted
- endothelial cells
- pulmonary embolism
- microbial community
- gram negative
- multiple sclerosis
- antimicrobial resistance
- risk assessment