Different Conditions for the Modification of Polycaprolactone Films with L-Arginine.
Yuliya NashchekinaAlina ChabinaNatalia A MikhailovaAlexey V NashchekinPublished in: International journal of molecular sciences (2020)
Poly-ε-caprolactone (PCL) is a biodegradable polymer used in regenerative medicine. Mesenchymal stem cells (MSCs) play an important role in the regeneration of different tissues. The hydrophobicity and neutrality of a PCL surface reduce MSCs' adhesion and proliferation. In this study, PCL films were treated with arginine to improve surface hydrophilicity. The influences of arginine concentration, temperature, and solvent on PCL surface properties were investigated. PCL films treated with a solution of arginine in isopropyl alcohol were found to have the maximum number of amino groups. The greatest number of cells, 2 h after seeding, adhered to such films. It was shown that amino groups affect the interaction of cells with a modified surface and the hydrolysis reaction after treatment with isopropyl alcohol promotes the formation of adhesive focal contacts. Hence, our results illustrate that functional groups on the PCL surface after arginine solution treatment regulate MSC adhesion and focal contact formation.
Keyphrases
- mesenchymal stem cells
- nitric oxide
- induced apoptosis
- room temperature
- umbilical cord
- cell cycle arrest
- stem cells
- amino acid
- gene expression
- bone marrow
- escherichia coli
- carbon nanotubes
- pseudomonas aeruginosa
- oxidative stress
- biofilm formation
- endoplasmic reticulum stress
- cell death
- ionic liquid
- alcohol consumption
- pi k akt
- tissue engineering
- high speed
- atomic force microscopy