Cryo scanning transmission x-ray microscope optimized for spectrotomography.
A F G LeontowichR BergC N RegierD M TaylorJ WangD BeauregardJ GeilhufeJ SwirskyJ WuC KarunakaranAdam P HitchcockStephen G UrquhartPublished in: The Review of scientific instruments (2018)
A cryo scanning transmission X-ray microscope, the cryo-STXM, has been designed and commissioned at the Canadian Light Source synchrotron. The instrument is designed to operate from 100 to 4000 eV (λ = 12.4 - 0.31 nm). Users can insert a previously frozen sample, through a load lock, and rotate it ±70° in the beam to collect tomographic data sets. The sample can be maintained for extended periods at 92 K primarily to suppress radiation damage and a pressure on the order of 10-9 Torr to suppress sample contamination. The achieved spatial resolution (30 nm) and spectral resolution (0.1 eV) are similar to other current soft X-ray STXMs, as demonstrated by measurements on known samples and test patterns. The data acquisition efficiency is significantly more favorable for both imaging and tomography. 2D images, 3D tomograms, and 4D chemical maps of automotive hydrogen fuel cell thin sections are presented to demonstrate current performance and new capabilities, namely, cryo-spectrotomography in the soft X-ray region.
Keyphrases
- electron microscopy
- high resolution
- electronic health record
- photodynamic therapy
- optical coherence tomography
- deep learning
- big data
- risk assessment
- single molecule
- magnetic resonance imaging
- stem cells
- mass spectrometry
- machine learning
- convolutional neural network
- cell therapy
- data analysis
- dual energy
- drinking water
- climate change
- patient reported outcomes
- heavy metals
- cone beam