Rosmarinic acid protects on rat bone marrow mesenchymal stem cells from hydrogen peroxide-induced apoptosis.
Li-Zhen LinHuan-Huan ChenZhou-Xi LeiYun-Rong LiChun-Hua ZhouYue-Chun HuangGang WeiPublished in: Journal of Asian natural products research (2018)
To investigate the anti-oxidant activities and mechanism of rosmarinic acid (RA) on rat bone marrow mesenchymal stem cells (rBMSCs) from ischemia-induced apoptosis in vitro, which was established using H2O2-damage and analyzed for cell viability, cell apoptosis, ROS, morphological changes, and levels of apoptosis proteins. Pretreatment with RA significantly suppressed the generation of ROS, protected the morphological changes of cells, decrease the ratio of cell apoptosis, down-regulated the level of caspase-3, caspase-9, Bax/Bcl-2, and up-regulated the level of p-PI3K. These findings suggest that RA may protect rBMSCs from H2O2-induced apoptosis by partly regulating PI3K/Akt signaling pathway and can be developed as a potential anti-apoptotic agent for therapy in cardiovascular diseases.
Keyphrases
- induced apoptosis
- signaling pathway
- pi k akt
- cell cycle arrest
- oxidative stress
- hydrogen peroxide
- cell death
- bone marrow
- rheumatoid arthritis
- cell proliferation
- endoplasmic reticulum stress
- dna damage
- disease activity
- cardiovascular disease
- nitric oxide
- transcription factor
- epithelial mesenchymal transition
- ankylosing spondylitis
- stem cells
- mesenchymal stem cells
- reactive oxygen species
- type diabetes
- metabolic syndrome
- systemic lupus erythematosus
- climate change