Core-shell magnetic porous organic polymer for magnetic solid-phase extraction of fluoroquinolone antibiotics in honey samples followed by HPLC with fluorescence detection.

By monomer-mediated in-situ growth synthesis strategy, with hydroquinone and 1,3,5-tris(4-aminophenyl)benzene as monomers, a core-shell magnetic porous organic polymer was synthesized through simple azo reaction. Based on this, a magnetic solid-phase extraction-HPLC-fluorescence detection method was proposed for the analysis of fluoroquinolones in honey sample. With ofloxacin, ciprofloxacin, enrofloxacin, lomefloxacin, and difloxacin as target analytes, factors affecting the extraction efficiency have been optimized. The LODs were 1.5-5.4 ng/L (corresponding to 0.23-0.81 μg/kg in honey). The linear range was 0.005-20 μg/L for difloxacin, 0.01-20 μg/L for ofloxacin, ciprofloxacin and lomefloxacin, and 0.02-20 μg/L for enrofloxacin. The enrichment factor is 84.4-91.7-fold with high extraction efficiency of 84.4-91.7%. The method was assessed by the analysis of target fluoroquinolones in honey samples, and the recoveries for the spiked samples were 79.3-95.8%. The results indicate that the established magnetic solid-phase extraction -HPLC-fluorescence detection method is efficient for the analysis of trace fluoroquinolones in honey. This article is protected by copyright. All rights reserved.