Surface plasmon resonance aptasensor for soluble ICAM-1 protein in blood samples.
Ali Doğan DursunSoner DoganMurat KavrukB Busra TasbasiMert SudagidanM Deniz YilmazBayram YilmazVeli Cengiz OzalpBilge Guvenc TunaPublished in: The Analyst (2022)
Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml -1 , which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.
Keyphrases
- label free
- sensitive detection
- magnetic nanoparticles
- quantum dots
- gold nanoparticles
- end stage renal disease
- loop mediated isothermal amplification
- protein protein
- ejection fraction
- binding protein
- squamous cell carcinoma
- cardiovascular disease
- newly diagnosed
- chronic kidney disease
- amino acid
- small cell lung cancer
- papillary thyroid
- type diabetes
- cystic fibrosis
- energy transfer
- escherichia coli
- young adults
- lymph node metastasis