Differentiation of Disaccharide Isomers via a Combination of IR and UV Photodissociation Mass Spectrometry.

This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers. The double-beam photodissociation method described here distinguished composition-, configuration- and connectivity disaccharide isomers successfully. Compared to the data accumulation method based on separated IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.