Practical two-photon-absorption cross sections and spectra of eosin and hematoxylin.
Jacopo ParraviciniAlessandra TomaselliElton HasaniDaniela TomassiniNorberto ManfrediLuca TartaraPublished in: Journal of biophotonics (2020)
We experimentally investigate the two-photon absorption cross sections and spectra of eosin and hematoxylin for applications in nonlinear microscopy. The experiments are carried out on pure samples of the two dyes in DI-water solvent with different concentrations, in the typical range employed in standard staining procedures. Nonlinear fluorescence is excited by a line-shaped beam emitted by a Ti:Sapphire mode-locked laser in the wavelength range from 740 to 880 nm and is detected through a microscope setup. The two-photon absorption spectral response is systematically analyzed and discussed. Finally, the staining is applied on biological tissue samples, which are imaged by two-photon microscopy. Our results show that the employed dyes are fully suitable for applications in nonlinear imaging.
Keyphrases
- single molecule
- living cells
- high resolution
- monte carlo
- optical coherence tomography
- high speed
- high throughput
- density functional theory
- flow cytometry
- photodynamic therapy
- magnetic resonance imaging
- ionic liquid
- staphylococcus aureus
- pseudomonas aeruginosa
- magnetic resonance
- fluorescence imaging
- molecular dynamics
- biofilm formation
- single cell
- neural network
- candida albicans