Selective Gas Chromatography Mass Spectrometry Method for Ultratrace Detection of Selenocyanate.
Enea PaglianoKelly L LeBlancZoltan MesterPublished in: Analytical chemistry (2019)
The recent interest in the determination of selenocyanate (SeCN-) in wastewater systems has spurred the development of analytical methods for its determination at the ultratrace level. Since most of the current procedures require complex and costly instrumental configurations, we have developed a simple and rapid gas chromatography tandem mass spectrometry (GC/MS/MS) method able to detect SeCN- in water samples with a LOD of 0.1 ng/g Se. A 1 mL volume of aqueous sample was buffered with sodium bicarbonate and treated with triethyloxonium tetrafluoroborate for conversion of the analyte into volatile EtSeCN. The derivatization yield was higher than 90%, and it could tolerate concentrations of chloride or sulfate up to 2%. The EtSeCN was extracted in chloroform and could be detected in electron ionization and also in negative chemical ionization mode with a further gain in signal-to-noise ratio by a factor of 2. The method was applied for the analysis of natural waters with quantitation of SeCN- in the low ng/g region. The Se13C15N- internal standard could be used for isotope dilution. Quantitative spike recoveries of 1 ng/g Se were obtained from seawater and river water, and 1 ng/g Se could be quantified within a standard uncertainty of 15%.
Keyphrases
- gas chromatography
- tandem mass spectrometry
- solid phase extraction
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- mass spectrometry
- simultaneous determination
- high resolution mass spectrometry
- high resolution
- ms ms
- air pollution
- real time pcr
- anaerobic digestion
- loop mediated isothermal amplification