Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.

Kevin Maafu JumaKenta MorimotoVishnu SharmaKirti SharmaRadhika BiyaniManish BiyaniTeisuke TakitaKiyoshi Yasukawa

Published in: Molecular biology reports (2024)

Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.

Keyphrases

sars cov
loop mediated isothermal amplification
respiratory syndrome coronavirus
real time pcr
protein protein
label free
binding protein
high throughput
single cell
sensitive detection
copy number
gram negative
dna methylation
antimicrobial resistance
human health