Single-cell RNA sequencing reveals chemokine self-feeding of myeloma cells promotes extramedullary metastasis.
Shuang GengJing WangXiannian ZhangJia-Jia ZhangFan WuYuhong PangYuping ZhongJianbin WangWenming WangXiaoqing LyuYanyi HuangHong-Mei JingPublished in: FEBS letters (2019)
In this study, we aimed to determine the mechanisms underlying the initial extramedullary translocation of myeloma cells from bone marrow into peripheral blood. We found that clonal circulating plasma cells (cPCs) are more frequently detected by flow cytometry in extramedullary plasmacytoma (EMP) patients and worsen their prognosis. It is technically much easier to collect single cPCs using FACS than it is to perform EMP biopsy. Therefore, combining EMP imaging with cPC detection may be a promising strategy for prognostic stratification. Here, using single-cell transcriptome analysis, we found that the chemokine CXCL12, a key molecule involved in CXCR4-dependent cell retention in the bone marrow, is abnormally upregulated in cPCs and might initially enable cPCs to evade bone marrow retention and translocate into the bloodstream.
Keyphrases
- single cell
- bone marrow
- rna seq
- induced apoptosis
- newly diagnosed
- flow cytometry
- mesenchymal stem cells
- peripheral blood
- cell cycle arrest
- high throughput
- end stage renal disease
- multiple myeloma
- chronic kidney disease
- high resolution
- endoplasmic reticulum stress
- cell death
- prognostic factors
- oxidative stress
- stem cells
- klebsiella pneumoniae
- cell proliferation
- fine needle aspiration
- fluorescence imaging