Excited-state locked amino analogues of the green fluorescent protein chromophore with a giant Stokes shift.

Snizhana O ZaitsevaDilara A FarkhutdinovaNadezhda S BaleevaAlexander Yu SmirnovMarina B ZagudaylovaAleksander M ShakhovArtyom A AstafievMikhail S BaranovAnastasia V Bochenkova

Published in: RSC advances (2019)

We design a novel class of excited-state locked GFP chromophores by introducing an amine group at the double exo -bond and a difluoroboryl bridge. We show that these chromophores intrinsically exhibit a very large Stokes shift of 1 eV. Further tuning through chemical modifications of their aryl substituents makes them environmentally sensitive.

Keyphrases

fluorescent probe
living cells
quantum dots
molecular docking
protein protein
amino acid
rare case
transition metal