Activatable fluorescent probes for hydrolase enzymes based on coumarin-hemicyanine hybrid fluorophores with large Stokes shifts.

Hiroyoshi FujiokaShin-Nosuke UnoMako Kamiya Ryosuke KojimaKai JohnssonYasuteru Urano

Published in: Chemical communications (Cambridge, England) (2020)

We show that the equilibrium of intramolecular spirocyclization of coumarin-hemicyanine hybrid fluorophores can be finely tuned by means of chemical modifications. We used this scaffold to develop activatable fluorescent probes with large Stokes shifts for γ-glutamyltranspeptidase and esterase.

Keyphrases

tissue engineering
fluorescent probe
living cells
molecular dynamics
molecular dynamics simulations
small molecule